Martin-Luther-Universität Halle-Wittenberg

Kontakt

Dr. Sabine Rosahl

Telefon: +49 (0) 345 5582 1440

Leibniz-Institut für Pflanzenbiochemie
Weinberg 3
06120 Halle (Saale)

Prof. Birgit Dräger

Telefon: +49 (0)345 55-25765

Institut für Pharmazie
Hoher Weg 8
06120 Halle (Saale)

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Teilprojekt 02

Defence and stress metabolism of potato

Sabine Rosahl (IPB), Birgit Dräger (MLU)

Hydroxycinnamic acid amides (HCAA) are the major phytoalexins in pathogen-infected potato leaves where they are supposed to act as antimicrobial and as cell wall enforcing compounds. It has recently been recognized that, in contrast to the Solanaceae-specific biosynthetic enzymes, members of the BAHD family are responsible for the synthesis of HCAA in other plants. In Arabidopsis, a gene, which encodes an agmatine coumaroyltransferase (AtACT) is activated in response to Alternaria brassicicola infection. We propose to perform a detailed biochemical characterization of AtACT in order to gain insight into the mechanism of acyl transfer and a better understanding of the flexibility of the enzyme and the plasticity of secondary product formation. By introducing the gene into potato plants, novel HCAA should be produced, thus enlarging the spectrum of antimicrobial HCAA in potato.

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